Murabhoritari yekuyedza yebazi reFederal State Budgetary Institution "Rosselkhoztsentr" muKrasnoyarsk Territory yakatanga rudzi rutsva rwekutsvagisa - kuongororwa uye kuzivikanwa kwezvipenyu zvakagadziridzwa genetically (GMOs) vachishandisa iyo polymerase chain reaction nzira munguva chaiyo.
Panharaunda yeRussian Federation, kuvapo kweGMO mukudya uye zviyo zvekudya kunodzorwa neTechnical Regulations yeCustoms Union TR CU 015/2011 "Pakuchengetedzwa kwezviyo"; saizvozvo, kutonga kwezviri mukati meGMOs muzviyo. zvinosungirwa. Uyewo, maererano nemutemo wekugadzirwa kwembeu (Federal Law No. 17.12.1997-FZ yaDecember 149, 11, yakagadziriswa musi waJune 2021, XNUMX), munharaunda yeRussian Federation inorambidzwa kushandiswa pakudyara (kudyara) mbeu. yezvirimwa zvine chirongwa chemajini chakashandurwa pachishandiswa genetic nzira engineering. Parizvino, mutemo mutsva "Pakugadzirwa kwembeu" Nhamba 454-FZ yakagamuchirwa (inotanga kushanda musi waSeptember 1, 2023), maererano neyo yose mbeu yembeu inofanira kuongororwa kune zvinyorwa zveGMO.
Chipenyu chine genetically modified organism chipenyu chine genotype yakasandurwa zvekugadzira pachishandiswa nzira dzemajini einjiniya kuitira kuti ipe hunhu hunodiwa. Mukurima, genetic engineering inoshandiswa kugadzira mhando nyowani dzezvirimwa zvinopokana nemamiriro asina kunaka ezvakatipoteredza uye zvipembenene, uye zvine kukura kuri nani uye kuravira hunhu.
Iyo yakanyanya kunaka nzira yekuona maGMO muzvigadzirwa zvekudya uye zviyo ndiyo chaiyo-nguva polymerase chain reaction (PCR) nzira. Iyi inzira inonzwisisika uye yakarurama kwazvo yakavakirwa pakudzokororwa kwakasarudzika kwechikamu cheDNA nucleic acid uchishandisa maenzayimu pasi pemamiriro ekunze (in vitro). PCR inokutendera kuti uwane muzvinyorwa zviri pasi pechidzidzo chikamu chidiki cheruzivo rwemajini rwuri mune yakatarwa kutevedzana kweDNA nucleotides yechero chipenyu pakati pehuwandu hukuru hwezvimwe zvikamu zveDNA uye woiwedzera kakawanda.
Murabhoritari yekuyedza yebazi reFederal State Budgetary Institution "Rosselkhoztsentr" muKrasnoyarsk Territory, basa rekubvisa DNA kubva muchirimwa uye kuita PCR rinoitwa pachishandiswa michina yazvino inosangana nezvinodiwa pakuronga PCR laboratory. Amplification (kuumbwa kwemakopi akawanda echromosomal DNA zvikamu) kunoitwa uchishandisa ANK-M amplifier, iyo inosanganisirwa murejista yekuyera zviridzwa zveArshin FSIS system. Chishandiso ichi chine thermoblock inochengetedza tembiricha inodiwa pakuita uye yakarongedzwa uchishandisa yakavakirwa-mukati microcomputer, uye ine fluorescence detector inorekodha chiratidzo chefluorescent kubva pamusanganiswa wekuita kuronga kuunganidzwa kwePCR chigadzirwa.
PCR inoitwa pachishandiswa reagent kits ine yakakwirira (inopfuura 90%) kushanda kwePCR reaction, yakanyanya kunzwisiswa uye hunyanzvi hwekuonekwa kweGMOs, inosangana nezvinodiwa nenyika dzepasirese yemiganhu yekuonekwa (LOD) uye muganho wehuwandu. (LOQ), ine internal positive control (VPK), iyo inobvumira kusabvisa mhedzisiro yakaipa.
Danho rekutanga rekuongorora kuona maGMO ndeye GMO kuongorora - chidzidzo chakazara chekuona chirimwa DNA uye kuona chaiwo ma genetic zvinhu - mamwe emitemo inoteedzana (vanosimudzira uye terminator) uye majini. Kana chimwe chezvinhu izvi chikaonekwa, mitsara yakagadziridzwa yemhando yemiti inotariswa.
Murabhoritari yekuyedza yebazi reFederal State Budgetary Institution "Rosselkhoztsentr" muKrasnoyarsk Territory, zvidzidzo zviri kuitwa kuona maGMO mukudya uye kudyisa zviyo uye mumbeu dzekurima. Basa iri rinoitwa nenyanzvi dzakadzidziswa pamusoro wenyaya "Nheyo dzekutarisa maGMOs muzvigadzirwa zvezvirimwa zvakabva pachishandiswa chaiyo-nguva PCR" kuFederal State Budgetary Institution "Federal Center for Assessment of the Safety and Quality of Grain and Its. Kugadzira Zvigadzirwa" mudunhu reMoscow.
Nekudaro, nyanzvi dzekuedza marabhoritari dzakabudirira kugona nzira nyowani, yemazuva ano uye inovimbisa yekuongorora zvidyarwa zveGMO zvemukati. Mune ramangwana, zvakarongwa kusanganisa nzira iyi muchikamu chekubvumidzwa kwema laboratori ekuyedza kuburikidza neRussia Accreditation system.